Mutant mice deficient in Ku70 exhibit early aging. Using several specific criteria of aging, the mutant mice were found to display the same aging signs as control mice, but at a considerably earlier chronological age.

2001-03-01

Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP) and Proximity Ligation Assay (PLA) (PLA) applications. This antibody reacts with Human samples. Supplied as 100 µL purified antibody (0.25 mg/mL). Function of DNA-protein kinase catalytic subunit during the early meiotic prophase without Ku70 and Ku86.

Ku70; Ku70, bridge and pillars domain superfamily; Ku70/Ku80, N-terminal alpha/beta; Ku70/Ku80 beta-barrel domain; Ku70/Ku80 C-terminal arm; UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information. Q23976; UniProt Anti-Ku70/XRCC6 Antibody PA1642. Uniprot Id: P12956: NCBI Gene Id: 2547: Species Of This Entry: Human: Gene Name: XRCC6 Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-25011. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human samples. Supplied as … The Ku70/XRCC6 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Ku70/XRCC6 protein expression profile in cells. The kit can be used for measuring the relative amounts of Ku70/XRCC6 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie.

IHC-plus™ XRCC6 / Ku70 Antibody LS-B5387 is a pathologist validated IHC antibody Mouse anti-Human XRCC6 / Ku70. Validated for ELISA,IF,IHC,WB. Tested on 20 paraffin-embedded human tissues.

14375 Ensembl ENSG00000196419 ENSMUSG00000022471 UniProt P12956 P23475 RefSeq (mRNA) NM_001469 NM_001288976 NM_001288977 NM_001288978 NM_010247 RefSeq (protein) NP_001275905 NP_001275906 NP_001275907 NP_001460 NP_001275905.1 NP_001460.1 NP_034377 Location (UCSC) Chr 22: 41.62 – 41.66 Mb Chr 15: 81.99 – 82.04 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Ku70 is a protein that 2007-01-23 · Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex (PubMed:25941166, PubMed:25670504, PubMed:11493912, PubMed:22442688). 2008-02-05 · Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex.

Ku70 is the 70 kDa subunit of the lupus Ku autoantigen. The lupus Ku autoantigen was identified in individuals with systemic lupus erythematosus (SLE) and related disorders. The Ku antigen is a heterodimer of Ku70 and Ku80. The Ku70/Ku80 complex functions as a single-stranded DNA-dependent ATP-dependent helicase.

Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-27538. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP) and Proximity Ligation Assay (PLA) (PLA) applications. This antibody reacts with Human samples. Supplied as 100 µL purified antibody (0.25 mg/mL). Function of DNA-protein kinase catalytic subunit during the early meiotic prophase without Ku70 and Ku86. Hamer G., Roepers-Gajadien H.L., van Duyn-Goedhart A., Gademan I.S., Kal H.B., van Buul P.P., Ashley T., de Rooij D.G. All components of the double-stranded DNA break (DSB) repair complex DNA-dependent protein kinase (DNA-PK), including Ku70, Ku86, and DNA-PK catalytic subunit (DNA-PKcs Actinomycin D induces histone gamma-H2AX foci and complex formation of gamma-H2AX with Ku70 and nuclear DNA helicase II. Mischo H.E., Hemmerich P., Grosse F., Zhang S. Formation of gamma-H2AX foci is a P. O.cellular response to genotoxic stress, such as DNA double strand breaks or stalled replication forks. Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-25915.

The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). Two of these proteins, Ku70 and Ku80, were identified by immunoblot analysis. The third polypeptide, which was identified by mass spectrometry analysis, is identical to poly(ADP-ribose) polymerase-1(PARP-1), a 113-kDa enzyme that functions as a sensor of DNA damage. 55K isoform of CDK9 associates with Ku70 and is involved in DNA repair. Liu H., Herrmann C.H., Chiang K., Sung T.L., Moon S.H., Donehower L.A., Rice A.P. Positive elongation factor b (P-TEFb) is a cellular protein kinase that is required for RNA polymerase II (RNAP II) … Invitrogen Anti-Ku70 Recombinant Monoclonal (JM61-31), Catalog # MA5-32645.
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Ku bunden till DNA.png. Kristallstruktur av mänsklig Ku bunden till DNA . Ku70 visas i lila, Ku80 i blått och DNA-strängen i grönt. Identifierare. Tillsammans, Ku70 och Ku80 utgör Ku-heterodimeren , vilken binder till DNA- dubbelsträngbrott ändar och krävs för den icke-homolog sammanfogning (NHEJ) Ku70 är ett känt Sirt1-substrat, vilket indikerar att målreglering av olika sirtuiner En homologimodell för humana Sirtl-rester 214-497 (UniProt-inträde Q96EB6) -3 ) till sekvenser i UniProt-databasen (SwissProt och TrEMBL-komponenter).

We have determined that Ku70, Ku86, p300, and CREB-binding protein (CBP) are ESE-1 interacting proteins. The Ku proteins have previously been shown to bind to breaks in DNA where they function to recruit additional proteins that promote DNA repair.
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KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement.

Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins.

anti-Ku 70 antibody is a Rabbit Polyclonal antibody recognizes Ku 70, which can be used for Phosphorylation of Ser-51 does not affect DNA repair. [UniProt]

Ku70 binding activity was localized to the C-terminal coiled-coil domain of nCLU. Leucine residues 357, 358, and 361 of nCLU were necessary for Ku70-nCLU interaction. The N- and C-terminal coiled-coil domains of nCLU interacted with each other, suggesting that the protein could dimerize or fold. IHC-plus™ XRCC6 / Ku70 Antibody LS-B5387 is a pathologist validated IHC antibody Mouse anti-Human XRCC6 / Ku70.

Oct 6, 2020 4941, Sequence mapping, 1, The Three-dimensional Structure of the C-terminal DNA-binding Domain of Human Ku70 The C-terminal region consists of a 5 kDa SAP domain (Ku70-SAP) which involved in DNA binding during NHEJ reaction. Expression, Ku70 is ubiquitously Homo sapiens KU70(XRCC6) and KU80(XRCC5) protein sequences were retrieved from.